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Boppart leads development of label-free microscopic techniques to visualize cancer cells

1/8/2020

Ananya Sen, Beckman Institute

The Biophotonics Imaging Lab at the Beckman Institute is interested in developing imaging techniques that investigate tissues without using any staining or labels. Led by Illinois ECE Professor Stephen Allen Boppart, Abel Bliss Professor of Engineering, they have developed a unique system using a laser source that can capture more information about a tissue compared to traditional imaging techniques. That system provides better visualization of breast cancer cells and their associated extracellular vesicles, small packages which are known to increase in number and be associated with cancer.

Stephen Allen Boppart
Stephen Allen Boppart
“We can acquire information about the structure and metabolism of the living tissue. The imaging system allows us to capture all this information simultaneously, allowing us to see many more details about the tissue, cells, and their functions than the current ways of imaging,” said Boppart who leads the lab, and is also a medical doctor.

The study “Label-Free Visualization and Characterization of Extracellular Vesicles in Breast Cancer” was published in the Proceedings of the National Academy of Sciences, and highlighted on the cover of the journal.

“Cells use these extracellular vesicles to communicate with each other, even under normal conditions. Tumor cells will alter these extracellular vesicles, and release more throughout the body. For this reason, they have the potential to be used as markers for cancer progression,” Boppart said.

Traditional visualization techniques are dependent on the use of labels, which involves a lot of effort. “Our key contribution is that we can visualize these vesicles in living tissues without perturbing the tissues or using any labels. We can see how these vesicles interact with each other and with the tumors,” said Sixian You, a bioengineering graduate research assistant in the Boppart lab.

A video of 3D visualization of the tumor microenvironment highlighting the presence of NAD(P)H rich scattered along the blood vessels.

The technique used by the lab involves using ultrashort laser pulses which interacts with the tissue samples. “There are two mechanisms involved in imaging. Some of the tissue components emit different kinds of fluorescence which comes in different colors. The other mechanism involves molecular structures that when aligned in a certain way, will give you an entirely different set of colors,” You said.

The researchers have also begun to characterize the contents of the vesicles using this technique. “We have found that the vesicles which have higher concentrations of NADPH molecules are highly correlated with tumor aggressiveness, and are often located around vessels. By characterizing the vesicles, we have a good shot at detecting cancerous changes at an earlier stage,” You said.

A second paper Real-Time Intraoperative Diagnosis by Deep Neural Network Driven Multiphoton Virtual Histology” was published in Precision Oncology and looks at combining the label-free imaging with deep learning techniques.

Currently, when surgeons remove tumors from patients, they analyze the tissue samples from the margins of the tumor to ensure that all the cancer tissue has been removed. Unfortunately, the pathology labs that analyze the samples can take up to a few days to complete their analysis.

“We are trying to use the label-free technique to look at the tissue right away in the surgical room,” You said. “After we get the images we use deep learning, which can be used to differentiate between cancerous and normal breast tissue.”

 

“We hope that this technique will be used for medical diagnosis and more clinical applications. The current techniques are useful but outdated and time-consuming. We think that there is a lot of new information that we can gain from this technology,” Boppart said.

 

“The system itself is somewhat expensive, but not unlike other high-end microscopes currently in use, and costs should come down in a few years as the laser technology matures,” Boppart said. “The other inherent challenge for us is that if you want high resolution images, you need to focus on smaller areas. However, when we look at the smaller areas, we can still get an adequate picture of what is going on in the tissue.”

 

Boppart is affiliated with the Beckman Institute

 


The paper “Label-Free Visualization and Characterization of Extracellular Vesicles in Breast Cancer” is online at doi.org/10.1073/pnas.1909243116

 

The paper “Real-Time Intraoperative Diagnosis by Deep Neural Network Driven Multiphoton Virtual Histology” can be found at https://www.nature.com/articles/s41698-019-0104-3

 

Check out the original article on the Beckman site.